Hotspot

psaA-ycf3

LSC · 43269-43922 · 654 bp

Back to Pancratium

Pi 0.0107

MarkerSeek score 64.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 7
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	50.0	50.0	57.9	58.0	833 bp	1.0	79.8
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	831 bp	1.0	79.0
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	760 bp	1.0	78.7
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	764 bp	1.0	78.4
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	759 bp	1.0	77.7