Hotspot

psbC-trnS-UGA

LSC · 36792-36913 · 122 bp

Back to Pancratium

Pi 0.0574

MarkerSeek score 82.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 7
pi: 0.0574
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTGGCTGAGTGGTTGATAG	55.0	55.0	58.2	58.0	235 bp	1.0	80.9
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.2	58.1	231 bp	1.0	80.7
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.2	58.1	232 bp	1.0	80.7
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	224 bp	1.0	80.7
psbC-trnS-UGA_p5	TTCCATGACCCCTCTTAGCT	GGTGGCTGAGTGGTTGATAG	50.0	55.0	57.7	58.0	227 bp	1.0	80.1