Hotspot

rpoB-trnC-GCA

LSC · 27337-28564 · 1228 bp

Back to Pancratium

Pi 0.00898

MarkerSeek score 50.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 11
pi: 0.00898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	52.6	50.0	56.8	57.5	1295-1299 bp	1.0	59.0
rpoB-trnC-GCA_p2	CCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	52.6	50.0	56.8	58.8	1332-1336 bp	1.0	57.0
rpoB-trnC-GCA_p3	CCCTCATTTCCATTCCGGA	GACACCCGGATTTGAACTGG	52.6	55.0	56.8	58.8	1318-1322 bp	1.0	56.5
rpoB-trnC-GCA_p4	CCCTCATTTCCATTCCGGA	CCGGATTTGAACTGGGGATA	52.6	50.0	56.8	57.0	1313-1317 bp	1.0	55.0
rpoB-trnC-GCA_p5	CCCTCATTTCCATTCCGGA	ATAAAGGATTTGCAGTCCCCC	52.6	47.6	56.8	58.0	1296-1300 bp	1.0	54.9