Hotspot

rps2-rpoC2

LSC · 16803-16966 · 164 bp

Back to Pancratium

Pi 0.0184

MarkerSeek score 63.70

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 3
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AACTCCAGCCTCCATCATCT	CGCCCCAAACAATTTCCATG	50.0	50.0	58.1	58.3	278-279 bp	1.0	78.1
rps2-rpoC2_p2	AGCCTCCATCATCTCTTCCA	CGCCCCAAACAATTTCCATG	50.0	50.0	57.8	58.3	272-273 bp	1.0	77.5
rps2-rpoC2_p3	TCCAGCCTCCATCATCTCTT	CGCCCCAAACAATTTCCATG	50.0	50.0	57.8	58.3	275-276 bp	1.0	77.5
rps2-rpoC2_p4	GCAGAAATGTAAGGGGCCAT	CGCCCCAAACAATTTCCATG	50.0	50.0	58.2	58.3	334-335 bp	1.0	77.0
rps2-rpoC2_p5	GAACTCCAGCCTCCATCATC	CGCCCCAAACAATTTCCATG	55.0	50.0	57.7	58.3	279-280 bp	1.0	76.9