Hotspot

trnS-GCU-trnG-GCC

LSC · 8610-9753 · 1144 bp

Back to Pancratium

Pi 0.0134

MarkerSeek score 55.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 15
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1207-1275 bp	1.0	57.5
trnS-GCU-trnG-GCC_p2	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1202-1270 bp	1.0	56.6
trnS-GCU-trnG-GCC_p3	GCTTTAGTCCACTCAGCCAT	TCGAACGAATCACACTTTTACC	50.0	40.9	57.6	57.0	1208-1276 bp	1.0	55.4
trnS-GCU-trnG-GCC_p4	AGTCCACTCAGCCATCTCTC	TCGAACGAATCACACTTTTACC	55.0	40.9	58.5	57.0	1203-1271 bp	1.0	54.6
trnS-GCU-trnG-GCC_p5	TAGTCCACTCAGCCATCTCT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.2	57.3	1203-1271 bp	1.0	54.1