Hotspot

ycf1

SSC · 126137-131896 · 5760 bp

Pi 0.0114

MarkerSeek score 55.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5760
variable_sites: 313
pi: 0.0114
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGACGAAAATCCGATTCTTGC	CAGACAAATTTATTACCGAGTTGGA	47.6	36.0	58.3	57.6	193 bp	1.0	48.0
ycf1_p2	CGACGAAAATCCGATTCTTGC	TCAGACAAATTTATTACCGAGTTGG	47.6	36.0	58.3	57.6	194 bp	1.0	48.0
ycf1_p3	CGACGAAAATCCGATTCTTGC	ACAAATTTATTACCGAGTTGGAACA	47.6	32.0	58.3	57.5	190 bp	1.0	48.0
ycf1_p4	CGACGAAAATCCGATTCTTGC	AGACAAATTTATTACCGAGTTGGA	47.6	33.3	58.3	56.4	192 bp	1.0	48.0
ycf1_p5	CGACGAAAATCCGATTCTTGC	CAGACAAATTTATTACCGAGTTGG	47.6	37.5	58.3	56.3	193 bp	1.0	48.0