Hotspot

ndhF

SSC · 112183-114408 · 2226 bp

Back to Panax

Pi 0.00647
MarkerSeek score 47.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2226
variable_sites
63
pi
0.00647
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GATGTCCTTGCCCACACTAG TTTTTACGAACAGGCGGGTC 55.0 50.0 58.0 58.8 2615-2639 bp 0.933333 74.0
ndhF_p2 GATGTCCTTGCCCACACTAG TTCGCTTTTTACGAACAGGC 55.0 45.0 58.0 57.3 2622-2644 bp 0.266667 47.4
ndhF_p3 GATGTCCTTGCCCACACTAG CTTTTTACGAACAGGCGGGT 55.0 50.0 58.0 58.8 2618-2640 bp 0.266667 46.6
ndhF_p4 GATGGAATCGCCCATTACGA TTCGCTTTTTACGAACAGGC 50.0 45.0 57.8 57.3 2788-2810 bp 0.266667 46.2
ndhF_p5 ACATGCCGAAGTGATGGAAA TTCGCTTTTTACGAACAGGC 45.0 45.0 57.8 57.3 2705-2727 bp 0.266667 46.0