Hotspot

clpP

LSC · 71937-73930 · 1994 bp

Pi 0.00767

MarkerSeek score 47.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1994
variable_sites: 64
pi: 0.00767
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	AACAGGGTCAGAGGGATCAA	50.0	50.0	58.0	58.0	2830-2881 bp	1.0	83.8
clpP_p2	CTGAACGAGTCGCACATACA	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2829-2880 bp	1.0	83.8
clpP_p3	CTGAACGAGTCGCACATACA	GATTCGGTATAGCCCCTCCT	50.0	55.0	58.0	58.1	2939-2990 bp	1.0	83.6
clpP_p4	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2862-2913 bp	1.0	83.6
clpP_p5	GATCTGAACGAGTCGCACAT	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.1	58.0	2832-2883 bp	1.0	83.3