Hotspot

rpl32-tRNA-Leu (UAG)

SSC · 115792-116605 · 814 bp

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Pi 0.0101
MarkerSeek score 57.50
Primer available yes
Label rpl32-tRNA-Leu (UAG)

Feature Metadata

label_name
rpl32-tRNA-Leu (UAG)
feature_type
igs
region
SSC
length_bp
814
variable_sites
42
pi
0.0101
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-tRNA-Leu (UAG)_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 934-959 bp 1.0 83.3
rpl32-tRNA-Leu (UAG)_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 935-960 bp 1.0 83.3
rpl32-tRNA-Leu (UAG)_p3 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 944-969 bp 1.0 82.3
rpl32-tRNA-Leu (UAG)_p4 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 923-948 bp 1.0 82.2
rpl32-tRNA-Leu (UAG)_p5 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 924-949 bp 1.0 82.2