Hotspot

rpl14-rpl16

LSC · 83037-83174 · 138 bp

Back to Panax

Pi 0.0153
MarkerSeek score 61.90
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
138
variable_sites
9
pi
0.0153
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 ACACATCAATTCTCGAGCCC TGAAATGGGTGGAGTAACAGA 50.0 42.9 58.0 56.6 295-328 bp 1.0 64.3
rpl14-rpl16_p2 CCGCTGTTATCCGCTACATT TGAAATGGGTGGAGTAACAGA 50.0 42.9 58.1 56.6 276-309 bp 1.0 63.7
rpl14-rpl16_p3 ACACATCAATTCTCGAGCCC ACCAGGTCGAATCCTTTATGA 50.0 42.9 58.0 56.4 313-346 bp 1.0 63.0
rpl14-rpl16_p4 TCGACGATTGCTAGCTCCTA TGAAATGGGTGGAGTAACAGA 50.0 42.9 58.0 56.6 326-358 bp 0.933333 60.7
rpl14-rpl16_p5 TCGACGATTGCTAGCTCCTA ACCAGGTCGAATCCTTTATGA 50.0 42.9 58.0 56.4 344-376 bp 0.933333 59.5