Hotspot

tRNA-Thr (UGU)-tRNA-Leu (UAA)

LSC · 48671-49466 · 796 bp

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Pi 0.01
MarkerSeek score 55.20
Primer available yes
Label tRNA-Thr (UGU)-tRNA-Leu (UAA)

Feature Metadata

label_name
tRNA-Thr (UGU)-tRNA-Leu (UAA)
feature_type
igs
region
LSC
length_bp
796
variable_sites
40
pi
0.01
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr (UGU)-tRNA-Leu (UAA)_p1 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 848-891 bp 1.0 82.8
tRNA-Thr (UGU)-tRNA-Leu (UAA)_p2 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 880-923 bp 1.0 81.7
tRNA-Thr (UGU)-tRNA-Leu (UAA)_p3 GCGATGCTCTAACCTCTGAG TAAGTCCGTAGCGTCTACCA 55.0 50.0 57.9 57.6 850-893 bp 1.0 81.5
tRNA-Thr (UGU)-tRNA-Leu (UAA)_p4 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 857 bp 0.066667 40.4
tRNA-Thr (UGU)-tRNA-Leu (UAA)_p5 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 889 bp 0.066667 39.5