Hotspot

psaA-ycf3

LSC · 43807-44517 · 711 bp

Back to Panax

Pi 0.00501
MarkerSeek score 57.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
711
variable_sites
20
pi
0.00501
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 870-889 bp 1.0 82.7
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT TATGGCGCTTACTCCTGGTA 50.0 50.0 57.9 57.9 842-861 bp 1.0 82.6
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT CGATCAAGCCGCTGAGTATT 50.0 50.0 57.9 58.1 872-891 bp 1.0 81.9
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 803-822 bp 1.0 81.8
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 799-818 bp 0.933333 78.6