Hotspot

tRNA-Glu (UUC)-tRNA-Thr (GGU)

LSC · 32600-33406 · 807 bp

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Pi 0.0162
MarkerSeek score 65.50
Primer available yes
Label tRNA-Glu (UUC)-tRNA-Thr (GGU)

Feature Metadata

label_name
tRNA-Glu (UUC)-tRNA-Thr (GGU)
feature_type
igs
region
LSC
length_bp
807
variable_sites
59
pi
0.0162
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 922-1046 bp 1.0 87.8
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p2 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 885-1009 bp 1.0 87.8
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p3 TTTCGTAGTACCCTACCCCC TACCGCTGAGTTAAAAGGGC 55.0 50.0 57.8 57.9 887-1011 bp 1.0 87.1
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p4 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 850-974 bp 1.0 87.1
tRNA-Glu (UUC)-tRNA-Thr (GGU)_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 863-987 bp 1.0 87.0