Hotspot

tRNA-Ser (GCU)-tRNA-Gly (UCC)

LSC · 9516-10242 · 727 bp

Back to Panax

Pi 0.0124
MarkerSeek score 56.50
Primer available yes
Label tRNA-Ser (GCU)-tRNA-Gly (UCC)

Feature Metadata

label_name
tRNA-Ser (GCU)-tRNA-Gly (UCC)
feature_type
igs
region
LSC
length_bp
727
variable_sites
39
pi
0.0124
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p1 ATTAGCAATCCGCCGCTTTA CAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.6 788-808 bp 1.0 58.8
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p2 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 786-806 bp 1.0 57.8
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p3 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 787-807 bp 1.0 55.9
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p4 GCTTTAGTCCACTCAGCCAT CAACGAATCACACTTTTACCACT 50.0 39.1 57.6 57.6 774-794 bp 1.0 55.9
tRNA-Ser (GCU)-tRNA-Gly (UCC)_p5 ATTAGCAATCCGCCGCTTTA CAACGAATCACACTTTTACCAC 45.0 40.9 58.0 56.2 788-808 bp 1.0 55.8