Hotspot

psbK-psbI

LSC · 8771-9178 · 408 bp

Back to Panax

Pi 0.0127
MarkerSeek score 56.50
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
408
variable_sites
24
pi
0.0127
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACGCCCCGGATCATTAGATA 50.0 50.0 57.9 58.1 526-536 bp 1.0 83.1
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG TACGCCCCGGATCATTAGAT 50.0 50.0 57.9 58.1 527-537 bp 1.0 83.1
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TTACGCCCCGGATCATTAGA 50.0 50.0 57.9 58.3 528-538 bp 1.0 81.3
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT ACGCCCCGGATCATTAGATA 45.0 50.0 58.3 58.1 523-533 bp 1.0 81.1
psbK-psbI_p5 TGGCAAGCTGCTGTAAGTTT TACGCCCCGGATCATTAGAT 45.0 50.0 58.3 58.1 524-534 bp 1.0 81.1