Hotspot

rps16-tRNA-Gln (UUG)

LSC · 6368-8163 · 1796 bp

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Pi 0.0112
MarkerSeek score 75.00
Primer available yes
Label rps16-tRNA-Gln (UUG)

Feature Metadata

label_name
rps16-tRNA-Gln (UUG)
feature_type
igs
region
LSC
length_bp
1796
variable_sites
100
pi
0.0112
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-tRNA-Gln (UUG)_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1809-1869 bp 1.0 86.6
rps16-tRNA-Gln (UUG)_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1824-1884 bp 1.0 84.8
rps16-tRNA-Gln (UUG)_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1823-1883 bp 1.0 84.8
rps16-tRNA-Gln (UUG)_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1813-1873 bp 1.0 81.9
rps16-tRNA-Gln (UUG)_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1839-1899 bp 1.0 81.7