Hotspot

tRNA-His (GUG)-psbA

LSC · 81-484 · 404 bp

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Pi 0.0178
MarkerSeek score 69.40
Primer available yes
Label tRNA-His (GUG)-psbA

Feature Metadata

label_name
tRNA-His (GUG)-psbA
feature_type
igs
region
LSC
length_bp
404
variable_sites
30
pi
0.0178
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-His (GUG)-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 471-480 bp 1.0 86.2
tRNA-His (GUG)-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 474-483 bp 1.0 86.1
tRNA-His (GUG)-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 485-494 bp 1.0 85.9
tRNA-His (GUG)-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 484-493 bp 1.0 85.9
tRNA-His (GUG)-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 486-495 bp 1.0 85.9