Hotspot

ycf45

LSC · 93193-94821 · 1629 bp

Back to Palmaria

Pi 0.0595

MarkerSeek score 76.70

Primer available yes

Label ycf45

Feature Metadata

label_name: ycf45
feature_type: gene
region: LSC
length_bp: 1629
variable_sites: 97
pi: 0.0595
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45_p1	GGTTCATTTGGTGCAGGAGA	GGAGAGGTGGTAGAGTTGGT	50.0	55.0	58.1	58.1	2468 bp	1.0	83.5
ycf45_p2	GGTTCATTTGGTGCAGGAGA	TGGAGAGGTGGTAGAGTTGG	50.0	55.0	58.1	58.1	2469 bp	1.0	83.5
ycf45_p3	GGTTCATTTGGTGCAGGAGA	TTTCAGCATCTTCATCCGGG	50.0	50.0	58.1	58.0	2304 bp	0.5	58.9
ycf45_p4	TGAGTTCAAATGAGTGCGCT	TTTCAGCATCTTCATCCGGG	45.0	50.0	58.1	58.0	2059 bp	0.5	58.7
ycf45_p5	ATGTGCCAACCATAGGTGAC	TTTCAGCATCTTCATCCGGG	50.0	50.0	57.9	58.0	2276 bp	0.5	58.6