Hotspot

orf441

LSC · 96999-98324 · 1326 bp

Back to Palmaria

Pi 0.0928

MarkerSeek score 78.10

Primer available yes

Label orf441

Feature Metadata

label_name: orf441
feature_type: gene
region: LSC
length_bp: 1326
variable_sites: 123
pi: 0.0928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf441_p1	CAGGGCGTTGCTCTTTATCT	GTCCTATCGCTTGCTTCCAA	50.0	50.0	58.0	58.0	2140-2141 bp	1.0	85.7
orf441_p2	CAGGGCGTTGCTCTTTATCT	CGGTCTCTTTCCAGCATTGA	50.0	50.0	58.0	57.9	1876-1877 bp	1.0	85.4
orf441_p3	TCGTGCGACTTGTTCATGAT	GTCCTATCGCTTGCTTCCAA	45.0	50.0	57.9	58.0	2246-2247 bp	1.0	85.1
orf441_p4	ACACATCGGAACATTGCGTA	GTCCTATCGCTTGCTTCCAA	45.0	50.0	57.9	58.0	2326-2327 bp	1.0	84.9
orf441_p5	CAGGGCGTTGCTCTTTATCT	ACTTTTTCTTGCGAACCCCT	50.0	45.0	58.0	57.9	1945 bp	0.5	59.2