Hotspot

orf402

LSC · 145528-146730 · 1203 bp

Back to Palmaria

Pi 0.0856

MarkerSeek score 79.80

Primer available yes

Label orf402

Feature Metadata

label_name: orf402
feature_type: gene
region: LSC
length_bp: 1203
variable_sites: 103
pi: 0.0856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf402_p1	GCTGACGTATTACACCATCAGA	TGAAACTTTCATCTCATACGGCT	45.5	39.1	58.0	58.2	1279-1282 bp	1.0	46.4
orf402_p2	AGCTGACGTATTACACCATCAG	TGAAACTTTCATCTCATACGGCT	45.5	39.1	58.0	58.2	1280-1283 bp	1.0	46.4
orf402_p3	GCTGACGTATTACACCATCAGA	TGAAACTTTCATCTCATACGGC	45.5	40.9	58.0	56.8	1279-1282 bp	1.0	46.4
orf402_p4	AGCTGACGTATTACACCATCAG	TGAAACTTTCATCTCATACGGC	45.5	40.9	58.0	56.8	1280-1283 bp	1.0	46.4
orf402_p5	AGCTGACGTATTACACCATCA	TGAAACTTTCATCTCATACGGCT	42.9	39.1	56.8	58.2	1280-1283 bp	1.0	46.4