Hotspot

cpcS

LSC · 141227-141685 · 459 bp

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Pi 0.113

MarkerSeek score 76.00

Primer available yes

Label cpcS

Feature Metadata

label_name: cpcS
feature_type: gene
region: LSC
length_bp: 459
variable_sites: 52
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cpcS_p1	TCCGAGGCTAAGAAAACCCT	ATTATGCGCGCGGATTAGAT	50.0	45.0	58.3	58.0	917 bp	0.5	57.1
cpcS_p2	CGCAACTCTCTGGGTTGTAT	ATTATGCGCGCGGATTAGAT	50.0	45.0	57.6	58.0	1176 bp	0.5	56.8
cpcS_p3	TCCGAGGCTAAGAAAACCCT	AGCTTGCCACAAGACCTAAC	50.0	50.0	58.3	58.1	1214 bp	0.5	56.4
cpcS_p4	CGCAACTCTCTGGGTTGTAT	AGCTTGCCACAAGACCTAAC	50.0	50.0	57.6	58.1	1473 bp	0.5	56.1
cpcS_p5	TTCCGAGGCTAAGAAAACCC	ATTATGCGCGCGGATTAGAT	50.0	45.0	57.5	58.0	918 bp	0.5	56.0