Hotspot

psaM-rrs

LSC · 187357-188235 · 879 bp

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Pi 0.198

MarkerSeek score 83.10

Primer available yes

Label psaM-rrs

Feature Metadata

label_name: psaM-rrs
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 171
pi: 0.198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-rrs_p1	TGCAACGAAAATTTGGCTGT	CGTCCGCCACTAAGGTAAAA	40.0	50.0	57.4	57.9	990-994 bp	1.0	83.9
psaM-rrs_p2	TGCAACGAAAATTTGGCTGT	AACCTTCGTTCGACTTGCAT	40.0	45.0	57.4	58.1	972-976 bp	1.0	83.6
psaM-rrs_p3	TGCAACGAAAATTTGGCTGT	CTTCGTTCGACTTGCATGTG	40.0	50.0	57.4	57.8	969-973 bp	1.0	82.8
psaM-rrs_p4	TGCAACGAAAATTTGGCTGT	TGCATGTGTTAGGCATACCG	40.0	50.0	57.4	58.3	957-961 bp	1.0	81.9
psaM-rrs_p5	TGCAACGAAAATTTGGCTGT	AAACCTTCGTTCGACTTGCA	40.0	45.0	57.4	58.3	973-977 bp	1.0	81.8