Hotspot

ycf80-ssrA

LSC · 156210-156974 · 765 bp

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Pi 0.0732
MarkerSeek score 75.60
Primer available yes
Label ycf80-ssrA

Feature Metadata

label_name
ycf80-ssrA
feature_type
igs
region
LSC
length_bp
765
variable_sites
56
pi
0.0732
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf80-ssrA_p1 AACTGCAACGACTCAAGGTT GAGGGCATGTAACTCAGTGG 45.0 55.0 58.0 58.0 1986-1990 bp 1.0 84.2
ycf80-ssrA_p2 AACTGCAACGACTCAAGGTT CGTTTTCCCGTGGCTTAGTA 45.0 50.0 58.0 57.9 1721-1725 bp 1.0 83.9
ycf80-ssrA_p3 AACTGCAACGACTCAAGGTT TACGTTTTCCCGTGGCTTAG 45.0 50.0 58.0 57.9 1723-1727 bp 1.0 83.9
ycf80-ssrA_p4 AGTCGAATCTGCTTGGCAAA GAGGGCATGTAACTCAGTGG 45.0 55.0 58.1 58.0 1508-1512 bp 1.0 83.9
ycf80-ssrA_p5 CCTGGAAATATGACCGTCCG GAGGGCATGTAACTCAGTGG 55.0 55.0 58.1 58.0 1661 bp 0.5 58.7