Hotspot

trnC(gca)-trnL(taa)

LSC · 152975-153013 · 39 bp

Back to Palmaria

Pi 0.154

MarkerSeek score 76.60

Primer available yes

Label trnC(gca)-trnL(taa)

Feature Metadata

label_name: trnC(gca)-trnL(taa)
feature_type: igs
region: LSC
length_bp: 39
variable_sites: 6
pi: 0.154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC(gca)-trnL(taa)_p1	ATCCTTTACCCCCAGTTCGA	GTCTACCATTCCACCACACC	50.0	55.0	58.0	57.9	97 bp	1.0	83.8
trnC(gca)-trnL(taa)_p2	GGTATGGCCAAGTGGTAAGG	GTCTACCATTCCACCACACC	55.0	55.0	57.9	57.9	130 bp	1.0	82.4
trnC(gca)-trnL(taa)_p3	GGTAAGGCAGAGGATTGCAA	GTCTACCATTCCACCACACC	50.0	55.0	57.9	57.9	117 bp	1.0	82.2
trnC(gca)-trnL(taa)_p4	TGCAAATCCTTTACCCCCAG	GTCTACCATTCCACCACACC	50.0	55.0	57.8	57.9	102 bp	1.0	82.0
trnC(gca)-trnL(taa)_p5	AGGCAGAGGATTGCAAATCC	GTCTACCATTCCACCACACC	50.0	55.0	58.2	57.9	113 bp	1.0	81.6