Hotspot

cfxQ-trnC(gca)

LSC · 152849-152903 · 55 bp

Back to Palmaria

Pi 0.127

MarkerSeek score 76.30

Primer available yes

Label cfxQ-trnC(gca)

Feature Metadata

label_name: cfxQ-trnC(gca)
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 7
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cfxQ-trnC(gca)_p1	ACAGTCAGAAGATATTATGAAAAGCA	CCTTACCACTTGGCCATACC	30.8	55.0	57.0	57.9	124 bp	1.0	44.5
cfxQ-trnC(gca)_p2	ACAGTCAGAAGATATTATGAAAAGCAG	CCTTACCACTTGGCCATACC	33.3	55.0	57.9	57.9	124 bp	1.0	44.5
cfxQ-trnC(gca)_p3	ACAGTCAGAAGATATTATGAAAAGCA	TTGCAATCCTCTGCCTTACC	30.8	50.0	57.0	57.9	137 bp	1.0	44.1
cfxQ-trnC(gca)_p4	ACAGTCAGAAGATATTATGAAAAGCA	TCGAACTGGGGGTAAAGGAT	30.8	50.0	57.0	58.0	157 bp	1.0	43.6
cfxQ-trnC(gca)_p5	ACAGTCAGAAGATATTATGAAAAGCAG	TCGAACTGGGGGTAAAGGAT	33.3	50.0	57.9	58.0	157 bp	1.0	43.6