Hotspot

psaD-chlB

LSC · 96147-96359 · 213 bp

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Pi 0.146

MarkerSeek score 75.60

Primer available yes

Label psaD-chlB

Feature Metadata

label_name: psaD-chlB
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 31
pi: 0.146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaD-chlB_p1	CCAGTACTCCCACCAAATGT	ACCTGCGTACATCCAGTATG	50.0	50.0	57.1	57.1	299-300 bp	1.0	73.8
psaD-chlB_p2	CCAGTACTCCCACCAAATGT	GGACCTGCGTACATCCAGTA	50.0	55.0	57.1	58.9	301-302 bp	1.0	73.8
psaD-chlB_p3	CCCACCAAATGTAGGAGAAGG	ACCTGCGTACATCCAGTATG	52.4	50.0	58.0	57.1	291-292 bp	1.0	72.8
psaD-chlB_p4	CCCACCAAATGTAGGAGAAGG	GGACCTGCGTACATCCAGTA	52.4	55.0	58.0	58.9	293-294 bp	1.0	72.8
psaD-chlB_p5	CACCAAATGTAGGAGAAGGCA	ACCTGCGTACATCCAGTATG	47.6	50.0	57.9	57.1	289-290 bp	1.0	72.3