Hotspot

trnS(tga)-psaD

LSC · 95269-95720 · 452 bp

Back to Palmaria

Pi 0.195

MarkerSeek score 76.60

Primer available yes

Label trnS(tga)-psaD

Feature Metadata

label_name: trnS(tga)-psaD
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 88
pi: 0.195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(tga)-psaD_p1	CCGCAAATTACACGTGCATT	GATGGTCGTGAAGCAGCTAA	45.0	50.0	58.0	58.0	1283 bp	0.5	59.7
trnS(tga)-psaD_p2	CCCGGATGAAGATGCTGAAA	GATGGTCGTGAAGCAGCTAA	50.0	50.0	58.0	58.0	732 bp	0.5	59.6
trnS(tga)-psaD_p3	ACCAACTCTACCACCTCTCC	GATGGTCGTGAAGCAGCTAA	55.0	50.0	58.1	58.0	568 bp	0.5	59.4
trnS(tga)-psaD_p4	CCGCAAATTACACGTGCATT	TACGCAATGTTCCGATGTGT	45.0	45.0	58.0	57.9	1881 bp	0.5	59.1
trnS(tga)-psaD_p5	ATAGCAACACAGTTCCGCAA	GATGGTCGTGAAGCAGCTAA	45.0	50.0	58.1	58.0	1297 bp	0.5	59.0