Hotspot

ycf1

SSC · 122762-128164 · 5403 bp

Back to Paeonia

Pi 0.00946

MarkerSeek score 48.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5403
variable_sites: 269
pi: 0.00946
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCAATTCTCTGCGACGTT	TCGATTCAGAAGAGTAAGCACC	45.0	45.5	57.3	57.9	150-183 bp	1.0	67.9
ycf1_p2	TTCTCAATTCTCTGCGACGT	TCGATTCAGAAGAGTAAGCACC	45.0	45.5	57.3	57.9	151-184 bp	1.0	67.9
ycf1_p3	TCTCAATTCTCTGCGACGTT	CGATTCAGAAGAGTAAGCACCA	45.0	45.5	57.3	58.2	149-182 bp	1.0	67.6
ycf1_p4	TTCTCAATTCTCTGCGACGT	CGATTCAGAAGAGTAAGCACCA	45.0	45.5	57.3	58.2	150-183 bp	1.0	67.6
ycf1_p5	TCTCAATTCTCTGCGACGTTT	TCGATTCAGAAGAGTAAGCACC	42.9	45.5	58.0	57.9	150-183 bp	1.0	65.5