Hotspot

ndhF

SSC · 110080-112290 · 2211 bp

Back to Paeonia

Pi 0.00911

MarkerSeek score 47.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2211
variable_sites: 96
pi: 0.00911
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGAGGGATCCACCGAAGAA	TCCACAATCGGATTGCGAAT	50.0	45.0	58.0	58.0	2843-2867 bp	0.961538	81.8
ndhF_p2	GGGAAAGCGAGGAAGAAACA	TCCACAATCGGATTGCGAAT	50.0	45.0	58.1	58.0	2912-2936 bp	0.961538	81.2
ndhF_p3	AGAATACCGTCACCCATCCT	TCCACAATCGGATTGCGAAT	50.0	45.0	57.8	58.0	2973-2997 bp	0.961538	80.6
ndhF_p4	AAGAGGGATCCACCGAAGAA	ATGAAACACAATCGGGCAGT	50.0	45.0	58.0	58.1	2869-2887 bp	0.538462	63.0
ndhF_p5	GGGAAAGCGAGGAAGAAACA	ATGAAACACAATCGGGCAGT	50.0	45.0	58.1	58.1	2938-2956 bp	0.538462	62.5