Hotspot

rps18-rpl20

LSC · 68332-68766 · 435 bp

Back to Paeonia

Pi 0.0166
MarkerSeek score 52.40
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
435
variable_sites
30
pi
0.0166
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 ACGAATGAATAGATTGACCCTGA GCCAATTGCTTCTTAATCGT 39.1 40.0 57.4 54.7 552-558 bp 0.538462 24.4
rps18-rpl20_p2 AGACGAATGAATAGATTGACCCT GCCAATTGCTTCTTAATCGT 39.1 40.0 57.1 54.7 554-560 bp 0.538462 24.4
rps18-rpl20_p3 GACGAATGAATAGATTGACCCTG GCCAATTGCTTCTTAATCGT 43.5 40.0 57.1 54.7 553-559 bp 0.538462 24.4
rps18-rpl20_p4 ACGAATGAATAGATTGACCCTGAA GCCAATTGCTTCTTAATCGT 37.5 40.0 58.0 54.7 552-558 bp 0.538462 24.4
rps18-rpl20_p5 ACGAATGAATAGATTGACCCTG GCCAATTGCTTCTTAATCGT 40.9 40.0 56.0 54.7 552-558 bp 0.538462 24.4