Hotspot

petA-psbJ

LSC · 63464-64295 · 832 bp

Back to Paeonia

Pi 0.0196

MarkerSeek score 84.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 86
pi: 0.0196
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CTGGACGGATTCCTCTTTGG	50.0	55.0	58.0	58.0	1054-1082 bp	1.0	89.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CGATACGACTGGACGGATTC	50.0	55.0	58.0	58.0	1062-1090 bp	1.0	89.5
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	ATACGACTGGACGGATTCCT	50.0	50.0	58.0	57.9	1060-1088 bp	1.0	89.2
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	CCTGTGATCGGGTTAATCGG	50.0	55.0	58.0	58.1	1010-1038 bp	1.0	89.2
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TGGTATTCCTGTGATCGGGT	50.0	50.0	58.0	58.1	1017-1045 bp	0.923077	85.6