Hotspot

accD-psaI

LSC · 59564-60097 · 534 bp

Back to Paeonia

Pi 0.0158

MarkerSeek score 55.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 36
pi: 0.0158
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGCTTACATTGCATTTGCGG	TACTAGACCCACCAAAGGCA	45.0	50.0	58.0	58.0	762-796 bp	1.0	87.9
accD-psaI_p2	TAGTGTGTGCTTCTGGAGGA	TACTAGACCCACCAAAGGCA	50.0	50.0	58.0	58.0	958-992 bp	1.0	87.1
accD-psaI_p3	TGGGATCTGTAGTAGGCGAG	TACTAGACCCACCAAAGGCA	55.0	50.0	58.0	58.0	1030-1064 bp	1.0	86.6
accD-psaI_p4	TGCTTACATTGCATTTGCGG	ACTTTCCGGTTCAGACATCG	45.0	50.0	58.0	57.9	991-1064 bp	0.538462	65.9
accD-psaI_p5	TAGTGTGTGCTTCTGGAGGA	ACTTTCCGGTTCAGACATCG	50.0	50.0	58.0	57.9	1187-1260 bp	0.538462	65.8