Hotspot

rps4-trnT-UGU

LSC · 46874-47249 · 376 bp

Back to Paeonia

Pi 0.0205

MarkerSeek score 56.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 31
pi: 0.0205
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTTTTGAAACGAGGCCCTC	CTCAGAGGTTAGAGCATCGC	47.4	55.0	56.0	57.9	450-476 bp	0.961538	62.3
rps4-trnT-UGU_p2	TTTTTGAAACGAGGCCCTC	GGTTCGATTCCGATAGCTGG	47.4	55.0	56.0	58.2	409-435 bp	0.961538	62.1
rps4-trnT-UGU_p3	TTTTTGAAACGAGGCCCTC	ATGGTCATCGGTTCGATTCC	47.4	50.0	56.0	57.8	418-444 bp	0.961538	61.8
rps4-trnT-UGU_p4	TTTTTGAAACGAGGCCCTC	CCGCTTAGCTCAGAGGTTAG	47.4	55.0	56.0	57.5	458-484 bp	0.961538	59.3
rps4-trnT-UGU_p5	TTTTTGAAACGAGGCCCTC	CAGAGGTTAGAGCATCGCAT	47.4	50.0	56.0	57.5	448-474 bp	0.961538	59.2