Hotspot

psbC-trnS-UGA

LSC · 36088-36303 · 216 bp

Back to Paeonia

Pi 0.0238

MarkerSeek score 49.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 11
pi: 0.0238
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGATCGCGATTTTGAACCT	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.0	58.1	339-344 bp	1.0	83.7
psbC-trnS-UGA_p2	CGATCGCGATTTTGAACCTG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	338-343 bp	1.0	83.1
psbC-trnS-UGA_p3	TCGATCGCGATTTTGAACCT	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.0	58.2	285-290 bp	1.0	83.0
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	322-327 bp	1.0	82.7
psbC-trnS-UGA_p5	TCGATCGCGATTTTGAACCT	GGTCTTTTGGAGAGATGGCC	45.0	55.0	58.0	58.2	363-368 bp	1.0	82.6