Hotspot

psbM-trnD-GUC

LSC · 30152-31238 · 1087 bp

Back to Paeonia

Pi 0.00909

MarkerSeek score 59.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 60
pi: 0.00909
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGGCTGATTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	35.0	50.0	54.0	58.5	1234-1248 bp	1.0	50.1
psbM-trnD-GUC_p2	TTTGGCTGATTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	33.3	50.0	54.9	58.5	1235-1249 bp	1.0	48.9
psbM-trnD-GUC_p3	TTTTGGCTGATTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	31.8	50.0	55.7	58.5	1236-1250 bp	1.0	47.2
psbM-trnD-GUC_p4	TTGGCTGATTGTTTTTACGT	GTTCAATTGGTCAGAGCACC	35.0	50.0	54.0	57.0	1235-1249 bp	1.0	45.9
psbM-trnD-GUC_p5	CATTTTGGCTGATTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	33.3	50.0	57.5	58.5	1238-1252 bp	1.0	45.7