Hotspot

rps4

LSC · 45391-45996 · 606 bp

Back to Paederia

Pi 0.0011

MarkerSeek score 47.00

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TTACTTCGCGTCGGAACTTT	ACTTCGCTTCATTTCGTTCAG	45.0	42.9	57.9	57.2	761 bp	1.0	64.6
rps4_p2	TTACTTCGCGTCGGAACTTT	CTTCGCTTCATTTCGTTCAGT	45.0	42.9	57.9	57.2	760 bp	1.0	64.6
rps4_p3	TTTACTTCGCGTCGGAACTT	ACTTCGCTTCATTTCGTTCAG	45.0	42.9	57.9	57.2	762 bp	1.0	64.6
rps4_p4	TTTACTTCGCGTCGGAACTT	CTTCGCTTCATTTCGTTCAGT	45.0	42.9	57.9	57.2	761 bp	1.0	64.6
rps4_p5	TTACTTCGCGTCGGAACTTT	TCGCTTCATTTCGTTCAGTT	45.0	40.0	57.9	56.0	758 bp	1.0	63.1