Hotspot

rbcL

LSC · 54342-55778 · 1437 bp

Back to Paederia

Pi 0.00278

MarkerSeek score 48.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1437
variable_sites: 6
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTAAAGTTCAA	50.0	45.0	57.1	57.4	1504 bp	1.0	67.7
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTAAAGTTCA	50.0	45.0	57.1	57.4	1505 bp	1.0	67.7
rbcL_p3	TTCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTAAAGTTCAA	47.6	45.0	57.8	57.4	1505 bp	1.0	65.6
rbcL_p4	TTCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTAAAGTTCA	47.6	45.0	57.8	57.4	1506 bp	1.0	65.6
rbcL_p5	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTAAAGTTCAA	50.0	42.9	57.1	58.1	1505 bp	1.0	64.3