Hotspot

petB

LSC · 74396-75830 · 1435 bp

Back to Paederia

Pi 0.00139

MarkerSeek score 59.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1435
variable_sites: 3
pi: 0.00139
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TATTTGGGGAGTTCGATCGC	TCCTCCTCCAAACGATAAATGA	50.0	40.9	57.8	56.9	1530-1532 bp	1.0	53.4
petB_p2	AGAGCCCCTATTTTGAGCCT	TCCTCCTCCAAACGATAAATGA	50.0	40.9	58.4	56.9	1554-1556 bp	1.0	52.1
petB_p3	TATTTGGGGAGTTCGATCGC	TTCCTCCTCCAAACGATAAATGA	50.0	39.1	57.8	57.6	1531-1533 bp	1.0	50.8
petB_p4	AGAGCCCCTATTTTGAGCCT	TTCCTCCTCCAAACGATAAATGA	50.0	39.1	58.4	57.6	1555-1557 bp	1.0	49.5
petB_p5	TATTTGGGGAGTTCGATCGC	CCTCCTCCAAACGATAAATGA	50.0	42.9	57.8	55.4	1529-1531 bp	1.0	49.4