Hotspot

ndhG

SSC · 117723-118253 · 531 bp

Back to Paederia

Pi 0.00126

MarkerSeek score 32.50

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTAATACATCGGTCGGGCAG	TCCGAGGCCGAATCAATTTT	55.0	45.0	57.9	57.9	1954 bp	1.0	78.7
ndhG_p2	CTAATACATCGGTCGGGCAG	TTCCGAGGCCGAATCAATTT	55.0	45.0	57.9	57.9	1955 bp	1.0	78.7
ndhG_p3	CTAATACATCGGTCGGGCAG	TTTCCGAGGCCGAATCAATT	55.0	45.0	57.9	57.9	1956 bp	1.0	78.7
ndhG_p4	CTAATACATCGGTCGGGCAG	AAAGCTGATCACACCTGAGC	55.0	50.0	57.9	58.2	1977 bp	1.0	78.2
ndhG_p5	CTAATACATCGGTCGGGCAG	CCGAGGCCGAATCAATTTTG	55.0	50.0	57.9	57.8	1953 bp	1.0	78.1