Hotspot

ndhA

SSC · 119187-121441 · 2255 bp

Back to Paederia

Pi 0.000887

MarkerSeek score 45.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2255
variable_sites: 3
pi: 0.000887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAGACAAGTCGTGAATCCAG	TCATTATGGGAGAAGTTGATCGT	47.6	39.1	57.7	57.4	2326-2327 bp	1.0	40.8
ndhA_p2	TGAGACAAGTCGTGAATCCA	TCATTATGGGAGAAGTTGATCGT	45.0	39.1	56.5	57.4	2326-2327 bp	1.0	40.1
ndhA_p3	GAGACAAGTCGTGAATCCAGA	TCATTATGGGAGAAGTTGATCGT	47.6	39.1	57.4	57.4	2325-2326 bp	1.0	40.1
ndhA_p4	TGAGACAAGTCGTGAATCCAG	TGGGAGAAGTTGATCGTTAA	47.6	40.0	57.7	54.0	2320-2321 bp	1.0	40.1
ndhA_p5	TGAGACAAGTCGTGAATCCAG	TCATTATGGGAGAAGTTGATCGTT	47.6	37.5	57.7	58.0	2326-2327 bp	1.0	40.1