Hotspot

matK

LSC · 1903-3420 · 1518 bp

Back to Paederia

Pi 0.000878

MarkerSeek score 46.80

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 2
pi: 0.000878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACAAGTACAAGGCTTCAGAATG	CCGCCTCCGAGGTATCTATT	40.9	55.0	56.6	58.5	1653 bp	1.0	49.5
matK_p2	ACAAGTACAAGGCTTCAGAA	CCGCCTCCGAGGTATCTATT	40.0	55.0	54.5	58.5	1653 bp	1.0	48.6
matK_p3	ACAAGTACAAGGCTTCAGAATG	CGCCTCCGAGGTATCTATTC	40.9	55.0	56.6	57.1	1652 bp	1.0	45.7
matK_p4	ACAAGTACAAGGCTTCAGAAT	CCGCCTCCGAGGTATCTATT	38.1	55.0	55.1	58.5	1653 bp	1.0	45.5
matK_p5	CCATTTACAAGTACAAGGCTTCA	CCGCCTCCGAGGTATCTATT	39.1	55.0	57.0	58.5	1659 bp	1.0	44.8