Hotspot

atpB

LSC · 52252-53748 · 1497 bp

Back to Paederia

Pi 0.000891
MarkerSeek score 46.80
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
LSC
length_bp
1497
variable_sites
2
pi
0.000891
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 AATAGGGGCATGGTTGGGTA ATCGACTCGCTATCGGCTAT 50.0 50.0 58.4 58.3 1695 bp 1.0 74.9
atpB_p2 AGGGGCATGGTTGGGTAATA ATCGACTCGCTATCGGCTAT 50.0 50.0 58.4 58.3 1692 bp 1.0 74.9
atpB_p3 ATAGGGGCATGGTTGGGTAA ATCGACTCGCTATCGGCTAT 50.0 50.0 58.4 58.3 1694 bp 1.0 74.9
atpB_p4 TAGGGGCATGGTTGGGTAAT ATCGACTCGCTATCGGCTAT 50.0 50.0 58.4 58.3 1693 bp 1.0 74.9
atpB_p5 AGGGGCATGGTTGGGTAATA CGACTCGCTATCGGCTATTT 50.0 50.0 58.4 57.7 1690 bp 1.0 74.3