Hotspot

rps15-ycf1

SSC · 123008-123380 · 373 bp

Back to Paederia

Pi 0.00357
MarkerSeek score 30.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
373
variable_sites
2
pi
0.00357
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ACCTCTGTTTTCGTCTTCCA CCGTGGTTGAAAAGTCATTGA 45.0 42.9 56.7 57.0 436 bp 1.0 54.2
rps15-ycf1_p2 ACCTCTGTTTTCGTCTTCCA TCCGTGGTTGAAAAGTCATTG 45.0 42.9 56.7 57.0 437 bp 1.0 54.2
rps15-ycf1_p3 ACCTCTGTTTTCGTCTTCCA TGTATCCGTGGTTGAAAAGTCA 45.0 40.9 56.7 57.9 441 bp 1.0 53.4
rps15-ycf1_p4 ACCTCTGTTTTCGTCTTCCAA CCGTGGTTGAAAAGTCATTGA 42.9 42.9 57.4 57.0 436 bp 1.0 52.1
rps15-ycf1_p5 ACCTCTGTTTTCGTCTTCCAA TCCGTGGTTGAAAAGTCATTG 42.9 42.9 57.4 57.0 437 bp 1.0 52.1