Hotspot

rpl16-rps3

LSC · 82090-82220 · 131 bp

Back to Paederia

Pi 0.0102
MarkerSeek score 51.60
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
131
variable_sites
2
pi
0.0102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AAACCAACGAGTCACACACT TGTTGCTATACAGTTCGAACCA 45.0 40.9 57.6 57.7 229 bp 1.0 59.4
rpl16-rps3_p2 AACGAGTCACACACTAAGCA TGTTGCTATACAGTTCGAACCA 45.0 40.9 57.1 57.7 224 bp 1.0 55.6
rpl16-rps3_p3 ACCAACGAGTCACACACTAAG TGTTGCTATACAGTTCGAACCA 47.6 40.9 57.9 57.7 227 bp 1.0 53.9
rpl16-rps3_p4 ACGAGTCACACACTAAGCAT TGTTGCTATACAGTTCGAACCA 45.0 40.9 56.9 57.7 223 bp 1.0 53.8
rpl16-rps3_p5 AAACCAACGAGTCACACACT GTTGCTATACAGTTCGAACCA 45.0 42.9 57.6 56.0 228 bp 1.0 53.6