Hotspot

trnE-UUC-trnT-GGU

LSC · 30636-31549 · 914 bp

Back to Paederia

Pi 0.000729

MarkerSeek score 46.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 1
pi: 0.000729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1055 bp	1.0	78.8
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	1034 bp	1.0	78.3
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	1031 bp	1.0	78.1
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	GGAGCCCCTTATCGGATTTG	55.0	55.0	57.8	58.1	1074 bp	1.0	78.0
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	996 bp	1.0	77.8