Hotspot

trnK-UUU-rps16

LSC · 4149-4912 · 764 bp

Back to Paederia

Pi 0
MarkerSeek score 46.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
764
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTCGTGGTCTTCCAAACCTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.0 58.0 986-989 bp 1.0 79.9
trnK-UUU-rps16_p2 GTCGTGGTCTTCCAAACCTT CTTGAAAGGGGTGCTCAACT 50.0 50.0 58.0 58.0 982-985 bp 1.0 79.8
trnK-UUU-rps16_p3 GTCGTGGTCTTCCAAACCTT GGAGCTTCGCCCTAATCAAA 50.0 50.0 58.0 58.0 917-920 bp 1.0 79.7
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 904-907 bp 1.0 78.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 903-906 bp 1.0 78.9