Hotspot

ndhA

SSC · 124446-126597 · 2152 bp

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Pi 0.0116

MarkerSeek score 63.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2152
variable_sites: 25
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCATAATTCATGAACCCAGT	GCCCACCGGGTTTTATCAAT	39.1	50.0	57.8	58.5	2390-2392 bp	1.0	51.1
ndhA_p2	ACCCAGTTATCATAGGGAACA	GCCCACCGGGTTTTATCAAT	42.9	50.0	55.7	58.5	2374-2376 bp	1.0	50.6
ndhA_p3	GAACCCAGTTATCATAGGGAACA	GCCCACCGGGTTTTATCAAT	43.5	50.0	57.6	58.5	2376-2378 bp	1.0	49.7
ndhA_p4	TGAACCCAGTTATCATAGGGAAC	GCCCACCGGGTTTTATCAAT	43.5	50.0	57.6	58.5	2377-2379 bp	1.0	49.7
ndhA_p5	AACCCAGTTATCATAGGGAACA	GCCCACCGGGTTTTATCAAT	40.9	50.0	56.5	58.5	2375-2377 bp	1.0	48.8