Hotspot

rpl2-rpl23

IRb · 89556-89579 · 24 bp

Back to Pachysandra

Pi 0.0417

MarkerSeek score 76.50

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 24
variable_sites: 1
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CACTTGACTGTCTACGGCTC	CGCTTCAACCGGGTTATTCT	55.0	50.0	58.0	58.3	134 bp	1.0	78.3
rpl2-rpl23_p2	GACTGTCTACGGCTCCATTG	CGCTTCAACCGGGTTATTCT	55.0	50.0	58.1	58.3	129 bp	1.0	77.9
rpl2-rpl23_p3	CACTTGACTGTCTACGGCTC	ATCATTACGCTTCAACCGGG	55.0	50.0	58.0	58.3	141 bp	1.0	77.7
rpl2-rpl23_p4	GACTGTCTACGGCTCCATTG	ATCATTACGCTTCAACCGGG	55.0	50.0	58.1	58.3	136 bp	1.0	77.3
rpl2-rpl23_p5	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	105 bp	1.0	77.2