Hotspot

rps2-rpoC2

LSC · 17164-17334 · 171 bp

Back to Pachysandra

Pi 0.0435

MarkerSeek score 68.60

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 7
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	TGTTCCAATATCTTCTTGTCA	ACGATACACCAGAACACTCG	33.3	50.0	52.5	57.4	209-219 bp	1.0	46.4
rps2-rpoC2_p2	TGTTCCAATATCTTCTTGTCA	CGATACACCAGAACACTCGT	33.3	50.0	52.5	57.4	208-218 bp	1.0	46.4
rps2-rpoC2_p3	GATGTTCCAATATCTTCTTGTCA	ACGATACACCAGAACACTCG	34.8	50.0	54.5	57.4	211-221 bp	1.0	46.3
rps2-rpoC2_p4	GATGTTCCAATATCTTCTTGTCA	CGATACACCAGAACACTCGT	34.8	50.0	54.5	57.4	210-220 bp	1.0	46.3
rps2-rpoC2_p5	TGTTCCAATATCTTCTTGTCA	TCCACGATACACCAGAACAC	33.3	50.0	52.5	57.3	212-222 bp	1.0	46.3