Hotspot

ycf1-ndhF

SSC · 111741-111782 · 42 bp

Back to Pachypleurum

Pi 0.203

MarkerSeek score 74.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 42
variable_sites: 12
pi: 0.203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CCCTGCCCACACTAGAAAAA	GTGGGCGCATCTCTTCTTAT	50.0	50.0	57.7	57.8	854-885 bp	1.0	79.8
ycf1-ndhF_p2	CCCTGCCCACACTAGAAAAA	GGGGAATAAGAGGATTGGCC	50.0	55.0	57.7	57.7	974-1005 bp	1.0	79.3
ycf1-ndhF_p3	CCCTGCCCACACTAGAAAAA	TGTTCGTTGGATCCCTAGGA	50.0	50.0	57.7	57.8	1328-1359 bp	1.0	78.8
ycf1-ndhF_p4	CCCTGCCCACACTAGAAAAA	AAAATCTGCAGGTAGTGGGC	50.0	50.0	57.7	58.2	868 bp	0.333333	49.8
ycf1-ndhF_p5	CTCATGAAGGCCTACCGTTG	AAAATCTGCAGGTAGTGGGC	55.0	50.0	58.3	58.2	166 bp	0.333333	49.3